Preisvergleich / Wohnen / Büro / Patch-Clamp Applications and Protocols, Sachbücher von Alan A. Boulton, Wolfgang Walz, Glen B. Baker
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Patch-Clamp Applications and Protocols, Sachbücher von Alan A. Boulton, Wolfgang Walz, Glen B. Baker

E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance ... Mehr erfahren

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E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and st.

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Lieferzeit:2-4 Werktage
Marke:Springer